Fruiting bodies are markedly stimulated by red and blue light but the exact role of the bacteriophytochromes that are encoded in the genomes of these species in controlling morphogenesis is unknown. Now, microscopically small crystals are sprayed into the ultrashort XFEL pulses. This method has been named serial femtosecond crystallography SFX. Steve Clouse sclouse nsf. Skip directly to content. Some links on this page may take you to non-federal websites.
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Northeastern Illinois University Street: Light-dependent formation of S. Publications Produced as a Result of this Research Note: More experiments are underway to push time-resolution to femtoseconds in order to capture the unique intermediates of BphP photocycle.
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Summary of the work conducted in my laboratory. We reported results from the last four years at about 20 lectures at workshops and conferences.
We are now able to establish methods at pulsed X-ray sources that allow us to investigate important proteins and enzymes with static and time-resolved crystallography and provide a tool for a better understanding of structural dynamics, catalysis and the molecular basis of life, specifically photomorphogenesis of myxobacteria. Their policies may differ from this site.
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Some full text articles may not yet be available without a charge during the embargo administrative interval. We investigated the mechanism of fruiting body formation in myxobacteria with respect to light and published S. We successfully determined the crystal structures of two BphPs from S. This proposal addresses the physiological role of neu photoreceptors known as bacteriophytochromes BphPs in non-photosynthetic myxobacteria.
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In the meantime, we determined the room-temperature structure of S. This project will investigate the role of bacteriophytochromes through integrated studies of myxobacterial genetics, high-throughput Neuu sequence analysis, time-resolved spectroscopy, and elucidation of bacteriophytochrome structures by X-ray crystallography.
This method has been named serial femtosecond crystallography SFX. When clicking on a Digital Object Identifier DOI number, you will be taken to an external site maintained by the publisher. Back to Previous Page. This project will advance the understanding of microbial light perception and the evolution of photoreceptors, engaging undergraduate and Master’s level students including members of groups under-represented in science in interdisciplinary research in microbial genetics, spectroscopy and structural biology.
We are currently developing genetic tools to knock out BphP genes in related M. Fruiting bodies are markedly stimulated by red and blue light but the exact role of the bacteriophytochromes that are encoded in the genomes of these species in controlling morphogenesis is unknown.
Cellular Dynamics and Function Program Officer: We collaborate nationally and internationally with leaders in the field and provide ample opportunity for our undergraduate desotop graduate students to lead experiments at the world?
In collaboration destkop Dr. Emina Stojkovic E-Stojkovic neiu. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content. Preliminary results from these time-resolved serial femtosecond crystallographic experiments were already published in Science Reports and are being currently drafted for another publication.
This project addresses the physiological role of red-light photoreceptors known as bacteriophytochromes in the non-photosynthetic myxobacteria Stigmatella aurantiaca and Cystobacter fuscus which form fruiting bodies under starvation conditions.
This Research in Undergraduate Institutions proposal will provide insights into fundamental, light-induced mechanisms in biological systems by addressing the role of photoreceptors in unique microorganisms, the myxobacteria, which are distinguished by unusual light-controlled morphogenesis of fruiting bodies. Most importantly, we were able to successfully apply for funding through an NSF EAGER award that enables us in part to continue this exciting research.
Steve Clouse sclouse nsf. Among prokaryotes, myxobacteria are distinguished by a unique multicellular stage in their life cycle in which fruiting bodies are formed.
The students learn how to purify protein and prepare macroscopic and microscopic crystals. Saint Louis Avenue City: Now, microscopically small crystals are sprayed into the ultrashort XFEL pulses. Photoreceptors are proteins that enable organisms to perceive and respond to light, an essential environmental signal; however, their physiological role in non-photosynthetic bacteria remains largely unknown. The intense X-ray beam destroys the crystals, yet a diffraction pattern is recorded before the crystals disintegrate.
Interestingly, we were able to demonstrate that S.